How much bacteriostatic water do I add to a 1.6 mg Thymosin Alpha-1 vial?

There is no single right amount. More water makes each draw bigger and easier to measure; less water keeps the volume small. With 2 mL, a 1.6 mg vial is 800 mcg/mL, so every 10 units on a U-100 insulin syringe holds 80 mcg. With 1 mL it is 1.6 mg/mL (160 mcg per 10 units). With 3 mL it is 533.3 mcg/mL (53.333 mcg per 10 units). With 5 mL it is 320 mcg/mL (32 mcg per 10 units).

How many units is 1 mg of Thymosin Alpha-1 from a 1.6 mg vial?

With 2 mL of water the vial is 800 mcg/mL, so 1 mg is 1.25 mL, or 125 units on a U-100 syringe, which is more than one 1 mL syringe holds. With a different water volume the units change but the amount does not. The amount is an example for the math, not a recommendation.

How many 1 mg draws are in a 1.6 mg Thymosin Alpha-1 vial?

1.6 (1.6 mg ÷ 1 mg), whatever volume of water you add. In practice a little is lost to the syringe dead space and the last drops in the vial.

Thymosin Alpha-1 1.6 mg Reconstitution Chart

Short answer: 2 mL of bacteriostatic water in a 1.6 mg Thymosin Alpha-1 vial makes 800 mcg/mL. On a U-100 insulin syringe (1 mL = 100 units), every 10 units then holds 80 mcg. More water gives a weaker solution and a bigger draw for the same amount; the amount in the vial stays 1.6 mg.

Water volume chart for a 1.6 mg vial

1.6 mg Thymosin Alpha-1 vial, U-100 insulin syringe.
Water added	Concentration	Per 10 units	Per 1 unit
1 mL	1.6 mg/mL	160 mcg	16 mcg
2 mL	800 mcg/mL	80 mcg	8 mcg
3 mL	533.3 mcg/mL	53.333 mcg	5.333 mcg
5 mL	320 mcg/mL	32 mcg	3.2 mcg

Syringe units by amount

The amounts below are examples to show the conversion, not doses. How much to take is a decision for you and your prescriber.

Units on a U-100 syringe from a 1.6 mg vial.
Water added	500 mcg	1 mg
1 mL	31.3 units	62.5 units
2 mL	62.5 units	over 1 mL
3 mL	93.8 units	over 1 mL
5 mL	over 1 mL	over 1 mL

A 1.6 mg vial holds 3.2 draws of 500 mcg, 1.6 draws of 1 mg, no matter how much water you add.

How far a 1.6 mg vial goes

Draws per vial depend only on the amount per draw, not on the water. The volume and units columns use 2 mL of water (800 mcg/mL). Example amounts only, not doses.

1.6 mg vial: draws, volume and units per example amount.
Amount per draw	Draws per vial	Volume per draw	Units per draw
250 mcg	6.4	0.313 mL	31.3 units
500 mcg	3.2	0.625 mL	62.5 units
1 mg	1.6	1.25 mL	over 1 mL

Real counts run a little lower: some liquid stays in the syringe and at the bottom of the vial.

1.6 mg next to other Thymosin Alpha-1 vial sizes

To get the same 800 mcg/mL from another vial size, scale the water with the vial. Same strength means the same units for the same amount, whichever vial it comes from.

Water for 800 mcg/mL, and 1 mg draws per vial, by vial size.
Vial	Water for 800 mcg/mL	1 mg draws per vial
1.6 mg (this page)	2 mL	1.6
5 mg	6.25 mL	5
10 mg	12.5 mL (over 10 mL)	10

The math

Concentration. 1.6 mg ÷ 2 mL = 800 mcg/mL
Volume to draw. amount ÷ 800 mcg/mL = mL
Syringe units. mL × 100 = units

For a different water volume or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.

Other Thymosin Alpha-1 vial sizes: Thymosin Alpha-1 5 mg · Thymosin Alpha-1 10 mg

Half-life, time to peak and sources are on the main Thymosin Alpha-1 guide.

Thymosin Alpha-1 1.6 mg reconstitution: how much water and how many units