[Peptide · NAD+ · 500 mg vial]

NAD+ 500 mg reconstitution: how much water and how many units

Water volume chart · Syringe units · Draws per vial

Short answer: 5 mL of bacteriostatic water in a 500 mg NAD+ vial makes 100 mg/mL. On a U-100 insulin syringe (1 mL = 100 units), every 10 units then holds 10 mg. More water gives a weaker solution and a bigger draw for the same amount; the amount in the vial stays 500 mg.

Water volume chart for a 500 mg vial

500 mg NAD+ vial, U-100 insulin syringe.
Water addedConcentrationPer 10 unitsPer 1 unit
2 mL250 mg/mL25 mg2.5 mg
5 mL100 mg/mL10 mg1 mg
10 mL50 mg/mL5 mg500 mcg

Syringe units by amount

The amounts below are examples to show the conversion, not doses. How much to take is a decision for you and your prescriber.

Units on a U-100 syringe from a 500 mg vial.
Water added25 mg50 mg100 mg
2 mL10 units20 units40 units
5 mL25 units50 units100 units
10 mL50 units100 unitsover 1 mL

A 500 mg vial holds 20 draws of 25 mg, 10 draws of 50 mg, 5 draws of 100 mg, no matter how much water you add.

The math

  1. Concentration. 500 mg ÷ 5 mL = 100 mg/mL
  2. Volume to draw. amount ÷ 100 mg/mL = mL
  3. Syringe units. mL × 100 = units

For a different water volume or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.

Other NAD+ vial sizes

NAD+ 100 mg · NAD+ 1,000 mg

Half-life, time to peak and sources are on the main NAD+ (injectable) guide.

Frequently asked questions

How much bacteriostatic water do I add to a 500 mg NAD+ vial?+
There is no single right amount. More water makes each draw bigger and easier to measure; less water keeps the volume small. With 5 mL, a 500 mg vial is 100 mg/mL, so every 10 units on a U-100 insulin syringe holds 10 mg. With 2 mL it is 250 mg/mL (25 mg per 10 units). With 10 mL it is 50 mg/mL (5 mg per 10 units).
How many units is 50 mg of NAD+ from a 500 mg vial?+
With 5 mL of water the vial is 100 mg/mL, so 50 mg is 0.5 mL, or 50 units on a U-100 syringe. With a different water volume the units change but the amount does not. The amount is an example for the math, not a recommendation.
How many 50 mg draws are in a 500 mg NAD+ vial?+
10 (500 mg ÷ 50 mg), whatever volume of water you add. In practice a little is lost to the syringe dead space and the last drops in the vial.