How much bacteriostatic water do I add to a 50 mg SS-31 vial?

There is no single right amount. More water makes each draw bigger and easier to measure; less water keeps the volume small. With 10 mL, a 50 mg vial is 5 mg/mL, so every 10 units on a U-100 insulin syringe holds 500 mcg. With 2 mL it is 25 mg/mL (2.5 mg per 10 units). With 3 mL it is 16.67 mg/mL (1.667 mg per 10 units). With 5 mL it is 10 mg/mL (1 mg per 10 units).

How many units is 1 mg of SS-31 from a 50 mg vial?

With 10 mL of water the vial is 5 mg/mL, so 1 mg is 0.2 mL, or 20 units on a U-100 syringe. With a different water volume the units change but the amount does not. The amount is an example for the math, not a recommendation.

How many 1 mg draws are in a 50 mg SS-31 vial?

50 (50 mg ÷ 1 mg), whatever volume of water you add. In practice a little is lost to the syringe dead space and the last drops in the vial.

SS-31 50 mg Reconstitution: BAC Water & Units

Short answer: 10 mL of bacteriostatic water in a 50 mg SS-31 vial makes 5 mg/mL. On a U-100 insulin syringe (1 mL = 100 units), every 10 units then holds 500 mcg. More water gives a weaker solution and a bigger draw for the same amount; the amount in the vial stays 50 mg.

Water volume chart for a 50 mg vial

50 mg SS-31 vial, U-100 insulin syringe.
Water added	Concentration	Per 10 units	Per 1 unit
2 mL	25 mg/mL	2.5 mg	250 mcg
3 mL	16.67 mg/mL	1.667 mg	166.667 mcg
5 mL	10 mg/mL	1 mg	100 mcg
10 mL	5 mg/mL	500 mcg	50 mcg

Syringe units by amount

The amounts below are examples to show the conversion, not doses. How much to take is a decision for you and your prescriber.

Units on a U-100 syringe from a 50 mg vial.
Water added	500 mcg	1 mg	2 mg
2 mL	2 units	4 units	8 units
3 mL	3 units	6 units	12 units
5 mL	5 units	10 units	20 units
10 mL	10 units	20 units	40 units

A 50 mg vial holds 100 draws of 500 mcg, 50 draws of 1 mg, 25 draws of 2 mg, no matter how much water you add.

The math

Concentration. 50 mg ÷ 10 mL = 5 mg/mL
Volume to draw. amount ÷ 5 mg/mL = mL
Syringe units. mL × 100 = units

For a different water volume or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.

Other SS-31 vial sizes

SS-31 10 mg

Half-life, time to peak and sources are on the main SS-31 (Elamipretide) guide.

SS-31 50 mg reconstitution: how much water and how many units

Water volume chart for a 50 mg vial

Syringe units by amount

The math

Other SS-31 vial sizes

Frequently asked questions