How much bacteriostatic water do I add to a 100 mg NAD+ vial?

There is no single right amount. More water makes each draw bigger and easier to measure; less water keeps the volume small. With 2 mL, a 100 mg vial is 50 mg/mL, so every 10 units on a U-100 insulin syringe holds 5 mg. With 5 mL it is 20 mg/mL (2 mg per 10 units). With 10 mL it is 10 mg/mL (1 mg per 10 units).

How many units is 50 mg of NAD+ from a 100 mg vial?

With 2 mL of water the vial is 50 mg/mL, so 50 mg is 1 mL, or 100 units on a U-100 syringe. With a different water volume the units change but the amount does not. The amount is an example for the math, not a recommendation.

How many 50 mg draws are in a 100 mg NAD+ vial?

2 (100 mg ÷ 50 mg), whatever volume of water you add. In practice a little is lost to the syringe dead space and the last drops in the vial.

NAD+ 100 mg Reconstitution: BAC Water & Units

Short answer: 2 mL of bacteriostatic water in a 100 mg NAD+ vial makes 50 mg/mL. On a U-100 insulin syringe (1 mL = 100 units), every 10 units then holds 5 mg. More water gives a weaker solution and a bigger draw for the same amount; the amount in the vial stays 100 mg.

Water volume chart for a 100 mg vial

100 mg NAD+ vial, U-100 insulin syringe.
Water added	Concentration	Per 10 units	Per 1 unit
2 mL	50 mg/mL	5 mg	500 mcg
5 mL	20 mg/mL	2 mg	200 mcg
10 mL	10 mg/mL	1 mg	100 mcg

Syringe units by amount

The amounts below are examples to show the conversion, not doses. How much to take is a decision for you and your prescriber.

Units on a U-100 syringe from a 100 mg vial.
Water added	25 mg	50 mg	100 mg
2 mL	50 units	100 units	over 1 mL
5 mL	over 1 mL	over 1 mL	over 1 mL
10 mL	over 1 mL	over 1 mL	over 1 mL

A 100 mg vial holds 4 draws of 25 mg, 2 draws of 50 mg, 1 draw of 100 mg, no matter how much water you add.

The math

Concentration. 100 mg ÷ 2 mL = 50 mg/mL
Volume to draw. amount ÷ 50 mg/mL = mL
Syringe units. mL × 100 = units

For a different water volume or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.

Other NAD+ vial sizes

NAD+ 500 mg · NAD+ 1,000 mg

Half-life, time to peak and sources are on the main NAD+ (injectable) guide.

NAD+ 100 mg reconstitution: how much water and how many units

Water volume chart for a 100 mg vial

Syringe units by amount

The math

Other NAD+ vial sizes

Frequently asked questions