How much bacteriostatic water do I add to a 2 mg AOD-9604 vial?

There is no single right amount. More water makes each draw bigger and easier to measure; less water keeps the volume small. With 2 mL, a 2 mg vial is 1 mg/mL, so every 10 units on a U-100 insulin syringe holds 100 mcg. With 1 mL it is 2 mg/mL (200 mcg per 10 units). With 3 mL it is 666.7 mcg/mL (66.667 mcg per 10 units). With 5 mL it is 400 mcg/mL (40 mcg per 10 units).

How many units is 250 mcg of AOD-9604 from a 2 mg vial?

With 2 mL of water the vial is 1 mg/mL, so 250 mcg is 0.25 mL, or 25 units on a U-100 syringe. With a different water volume the units change but the amount does not. The amount is an example for the math, not a recommendation.

How many 250 mcg draws are in a 2 mg AOD-9604 vial?

8 (2 mg ÷ 250 mcg), whatever volume of water you add. In practice a little is lost to the syringe dead space and the last drops in the vial.

AOD-9604 2 mg Reconstitution: BAC Water & Units

Short answer: 2 mL of bacteriostatic water in a 2 mg AOD-9604 vial makes 1 mg/mL. On a U-100 insulin syringe (1 mL = 100 units), every 10 units then holds 100 mcg. More water gives a weaker solution and a bigger draw for the same amount; the amount in the vial stays 2 mg.

Water volume chart for a 2 mg vial

2 mg AOD-9604 vial, U-100 insulin syringe.
Water added	Concentration	Per 10 units	Per 1 unit
1 mL	2 mg/mL	200 mcg	20 mcg
2 mL	1 mg/mL	100 mcg	10 mcg
3 mL	666.7 mcg/mL	66.667 mcg	6.667 mcg
5 mL	400 mcg/mL	40 mcg	4 mcg

Syringe units by amount

The amounts below are examples to show the conversion, not doses. How much to take is a decision for you and your prescriber.

Units on a U-100 syringe from a 2 mg vial.
Water added	125 mcg	250 mcg	500 mcg
1 mL	6.3 units	12.5 units	25 units
2 mL	12.5 units	25 units	50 units
3 mL	18.8 units	37.5 units	75 units
5 mL	31.3 units	62.5 units	over 1 mL

A 2 mg vial holds 16 draws of 125 mcg, 8 draws of 250 mcg, 4 draws of 500 mcg, no matter how much water you add.

The math

Concentration. 2 mg ÷ 2 mL = 1 mg/mL
Volume to draw. amount ÷ 1 mg/mL = mL
Syringe units. mL × 100 = units

For a different water volume or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.

Other AOD-9604 vial sizes

AOD-9604 5 mg

Half-life, time to peak and sources are on the main AOD-9604 guide.

AOD-9604 2 mg reconstitution: how much water and how many units

Water volume chart for a 2 mg vial

Syringe units by amount

The math

Other AOD-9604 vial sizes

Frequently asked questions