Short answer: 10 mL of bacteriostatic water in a 40 mg retatrutide vial makes 4 mg/mL. On a U-100 insulin syringe (1 mL = 100 units), every 10 units then holds 400 mcg. More water gives a weaker solution and a bigger draw for the same amount; the amount in the vial stays 40 mg.
| Water added | Concentration | Per 10 units | Per 1 unit |
|---|---|---|---|
| 2 mL | 20 mg/mL | 2 mg | 200 mcg |
| 3 mL | 13.33 mg/mL | 1.333 mg | 133.333 mcg |
| 5 mL | 8 mg/mL | 800 mcg | 80 mcg |
| 10 mL | 4 mg/mL | 400 mcg | 40 mcg |
The amounts below are examples to show the conversion, not doses. How much to take is a decision for you and your prescriber.
| Water added | 1 mg | 2 mg | 4 mg |
|---|---|---|---|
| 2 mL | 5 units | 10 units | 20 units |
| 3 mL | 7.5 units | 15 units | 30 units |
| 5 mL | 12.5 units | 25 units | 50 units |
| 10 mL | 25 units | 50 units | 100 units |
A 40 mg vial holds 40 draws of 1 mg, 20 draws of 2 mg, 10 draws of 4 mg, no matter how much water you add.
Draws per vial depend only on the amount per draw, not on the water. The volume and units columns use 10 mL of water (4 mg/mL). Example amounts only, not doses.
| Amount per draw | Draws per vial | Volume per draw | Units per draw |
|---|---|---|---|
| 500 mcg | 80 | 0.125 mL | 12.5 units |
| 1 mg | 40 | 0.25 mL | 25 units |
| 2 mg | 20 | 0.5 mL | 50 units |
| 4 mg | 10 | 1 mL | 100 units |
| 8 mg | 5 | 2 mL | over 1 mL |
Real counts run a little lower: some liquid stays in the syringe and at the bottom of the vial.
To get the same 4 mg/mL from another vial size, scale the water with the vial. Same strength means the same units for the same amount, whichever vial it comes from.
| Vial | Water for 4 mg/mL | 2 mg draws per vial |
|---|---|---|
| 5 mg | 1.25 mL | 2.5 |
| 10 mg | 2.5 mL | 5 |
| 15 mg | 3.75 mL | 7.5 |
| 20 mg | 5 mL | 10 |
| 30 mg | 7.5 mL | 15 |
| 40 mg (this page) | 10 mL | 20 |
| 50 mg | 12.5 mL (over 10 mL) | 25 |
| 60 mg | 15 mL (over 10 mL) | 30 |
40 mg ÷ 10 mL = 4 mg/mLamount ÷ 4 mg/mL = mLmL × 100 = unitsFor a different water volume or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.
Other retatrutide vial sizes: Retatrutide 5 mg · Retatrutide 10 mg · Retatrutide 15 mg · Retatrutide 20 mg · Retatrutide 30 mg · Retatrutide 50 mg · Retatrutide 60 mg
Half-life, time to peak and sources are on the main Retatrutide guide.