PE-22-28 Reconstitution Calculator, Units & Half-Life
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PE-22-28: reconstitution, units and half-life

Vial sizes · Worked example · Units chart · Half-life notes

What is PE-22-28?

PE-22-28 is a synthetic seven-amino-acid peptide made by French researchers as a shorter, longer-lasting version of spadin, a natural peptide that blocks the TREK-1 potassium channel in the brain. It has been studied only in cells and mice and is not approved for human use.

Also known as: PE 22-28, Spadin analog.

Common vial sizes

We could not confirm a standard vial size for PE-22-28, so check the label on yours. The 10 mg vial in the example below is only there to show the math.

Worked reconstitution example

Here is the math for a 10 mg vial mixed with 2 mL of bacteriostatic water, drawing 500 mcg with a U-100 insulin syringe (1 mL = 100 units).

  1. Concentration. 10 mg ÷ 2 mL = 5 mg/mL
  2. Volume to draw. 500 mcg ÷ 5 mg/mL = 0.1 mL
  3. Syringe units. 0.1 mL × 100 = 10 units
The amount here is an example to show the math, not a recommendation. How much to take is a decision for you and your prescriber.

Units chart at 5 mg/mL

10 mg vial + 2 mL water. Amounts are examples for the conversion, not doses.
AmountVolumeU-100 syringe
250 mcg0.05 mL5 units
500 mcg0.1 mL10 units
1 mg0.2 mL20 units

Same amount, different water volumes

Adding more water doesn't change how much PE-22-28 is in the vial. It only spreads it over more liquid, so each draw is bigger and easier to read.

500 mcg from a 10 mg vial.
Water addedConcentrationVolumeU-100 syringe
1 mL10 mg/mL0.05 mL5 units
2 mL5 mg/mL0.1 mL10 units
3 mL3.33 mg/mL0.15 mL15 units
5 mL2 mg/mL0.25 mL25 units

For any other vial size or amount, the free peptide reconstitution calculator works backwards from the amount you want and suggests a water volume that lands on a clean syringe mark.

What is known in people?

Nothing yet: we could not find any published study or registered trial that gave PE-22-28 to people. Its parent peptide, spadin, had antidepressant-like effects in mice (Mazella 2010). In the paper that introduced PE-22-28 (Djillani 2017), it blocked TREK-1 far more strongly than spadin in cells, had antidepressant-like effects in mouse behavior tests after 4 days, increased new nerve cells, and its effect lasted up to 23 hours in mice versus 7 hours for spadin. Mouse results often do not carry over to people.

PE-22-28 half-life and time to peak

  • Half-lifeNot listed
  • Time to peak (Tmax)Not listed

We could not find a published human half-life for this compound. Figures quoted online usually come from animal or cell studies, so we do not list one here.

How to track PE-22-28 in Dose Track

  1. Add PE-22-28 as a custom compound. Start typing its name and Dose Track fills in the details from its research library for you to check, and it sits next to everything else you take.
  2. Save your vial: enter the vial size and how much water you added. From then on every amount you log shows as syringe units, and the reconstitution planner suggests a water volume that lands on a clean mark.
  3. Log each shot. The app subtracts it from the vial, shows what is left, and warns you before you run out.
  4. Set reminders on your own schedule, whether that is daily, weekly or every few days.
  5. Your log is stored on your phone and no account is needed. Data only leaves it for features you choose, like AI answers and lab reading.

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Frequently asked questions

How much bacteriostatic water do I add to a 10 mg PE-22-28 vial?+
There is no single right amount. More water makes each draw bigger and easier to measure; less water makes it smaller. For example, 2 mL in a 10 mg vial gives 5 mg/mL, so 500 mcg is 0.1 mL, or 10 units on a U-100 insulin syringe. That amount is only an example for the math, not a recommendation. The free Dose Track reconstitution calculator works it out for any vial and amount.
How many units is 500 mcg of PE-22-28?+
It depends on the concentration in your vial. At 5 mg/mL (10 mg mixed with 2 mL), 500 mcg is 0.1 mL, which is 10 units on a U-100 insulin syringe, where 1 mL is 100 units. Divide the amount by the concentration to get mL, then multiply by 100.
What is the half-life of PE-22-28?+
We could not find a published human half-life for this compound. Figures quoted online usually come from animal or cell studies, so we do not list one here.
Can I track PE-22-28 in Dose Track?+
Yes. PE-22-28 is in Dose Track's research library, so when you add it as a custom compound its details are filled in for you to check. You can then log each shot next to your other compounds, save your vial and how much water you added so every amount shows in syringe units, see how much is left in the vial, and get reminders. Your log is stored on your phone and no account is needed. Data only leaves it for features you choose, like AI answers and lab reading.

Sources

  1. Djillani A, et al. Shortened spadin analogs display better TREK-1 inhibition, in vivo stability and antidepressant activity. Front Pharmacol. 2017;8:643.
  2. Mazella J, et al. Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design. PLoS Biol. 2010;8(4):e1000355.
  3. Djillani A, et al. Fighting against depression with TREK-1 blockers: past and future. A focus on spadin (review). Pharmacol Ther. 2019;194:185-198.

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